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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jul 16, 2025

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

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Long-lived SERS Matrix for Real-Time Biochemical Detection Using "Frozen" Transition State.

Kai Zhu¹, Tong Zhou¹, Peng Chen^1,2

¹Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing 210096, China.

|September 13, 2023

Summary

This summary is machine-generated.

This study introduces a novel superwettable-omniphobic lubricous porous SERS (SOLP-SERS) substrate for long-term bio-event tracking. The SOLP-SERS platform maintains analyte bioactivity and achieves femtomolar detection limits with stable signals for over 30 days.

Keywords:

biosensing liquid hotspots nanoparticle nanopore omniphobicity real time

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Area of Science:

Analytical Chemistry
Materials Science
Biotechnology

Background:

Maintaining analyte bioactivity is crucial for long-time tracking of biological events.
Existing detection methods often struggle with analyte stability over extended periods.

Purpose of the Study:

To develop a versatile surface-enhanced Raman scattering (SERS) platform for sensitive and stable long-term detection of biological analytes.
To demonstrate the capability of the platform in monitoring dynamic biological processes and detecting disease biomarkers.

Main Methods:

Fabrication of a superwettable-omniphobic lubricous porous SERS (SOLP-SERS) substrate.
Utilizing a 3D liquid "hotspots" matrix formed by confining liquids within nanoparticle gaps.
Confining analytes within liquid "hotspots" to preserve bioactivity during SERS detection.

Main Results:

Achieved limits of detection down to femtomolar levels for various molecules.
Demonstrated uniform and stable SERS signals over 30 days.
Successfully monitored Aβ peptide polymerization and detected exosomes from breast cancer cells.

Conclusions:

The SOLP-SERS substrate offers an ultra-long lifetime for liquid "hotspots", ensuring analyte bioactivity.
The platform provides ultrahigh sensitivity and signal stability for real-time biochemical sensing.
This technology has significant potential for advancing the development of next-generation biochemical sensors.