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Updated: Jun 28, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Ion-modulated interfacial fluorescence in droplet microfluidics using an ionophore-doped oil
Renjie Wang1, Nasrin Ghanbari Ghalehjoughi1, Xuewei Wang1
1Department of Chemistry, Virginia Commonwealth University, 1001 W. Main Street, Richmond, VA 23284, USA. wangx11@vcu.edu.
Abstract:
Fluorescence at the oil-water interface is used for chemical sensing in droplet microfluidics. Potassium ions in aqueous droplets are extracted into oil segments doped with an ionophore, a cation exchanger, and a cationic dye to expel the dye. When a low concentration of dye with a balanced solubility is used, it actively accumulates at the thin interface between oil and water instead of getting dissolved in the aqueous phase. The interfacial fluorescence is monitored distinct from the fluorescence in the oil sensor and the aqueous sample, allowing for highly sensitive and selective turn-on fluorescence sensing of ions.
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