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Characterization of Linear IgE-Binding Epitopes in Food Allergens
Changqi Liu1, Sahil Gupta2, Jing Zhao3
1School of Exercise and Nutritional Sciences, San Diego State University, San Diego, CA, USA. changqi.liu@sdsu.edu.
Abstract:
An IgE epitope is a part of an allergen that is capable of binding to IgE antibodies and eliciting an immune response. Identifying and characterizing human-allergy-relevant epitopes are important for diagnosis and prognosis of food allergy and development of immunotherapy treatments. This chapter describes the protocol for manual synthesis of overlapping peptides on a cellulose membrane and subsequent dot blotting of the peptides with allergic patients' IgE to map the linear IgE-binding epitopes in food allergens.
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