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Related Experiment Video

Updated: Jul 15, 2025

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Published on: February 15, 2021

Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses.

Yoshitaka J Sei¹, Myriam M Chaumeil², Ken Nakamura³

¹Gladstone Institute of Neurological Disease, Gladstone Institutes, San Francisco, CA 94158, USA.

|September 24, 2023

Summary

This summary is machine-generated.

This study introduces a cost-effective spatial transcriptomics protocol by mounting multiple mouse brains onto a single slide. This method conserves reagents and increases sample throughput for gene expression analysis.

Keywords:

Biotechnology and Bioengineering Genomics Neuroscience

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Related Experiment Videos

Last Updated: Jul 15, 2025

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Published on: February 15, 2021

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

Area of Science:

Neuroscience
Genomics
Molecular Biology

Background:

Spatial transcriptomics is a powerful technique for analyzing gene expression within its spatial context.
Current limitations include high costs and limited sample capacity per slide, hindering broader application.

Purpose of the Study:

To develop a protocol that increases sample throughput for spatial transcriptomics.
To reduce the cost and reagent consumption associated with spatial transcriptomics experiments.

Main Methods:

A novel protocol for mounting multiple frozen mouse brain sections onto a single spatial transcriptomics slide.
Conjoining frozen hippocampal sections from different brains into a single cryostat block.

Main Results:

The protocol enables multiple samples to be processed on a single slide, significantly improving efficiency.
Reduced reagent usage due to the cryostat block conjoining technique.

Conclusions:

This protocol offers a cost-effective solution for spatial transcriptomics.
It is compatible with existing spatial genomics platforms and applicable to various brain regions.