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Updated: Jul 15, 2025
14:18
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Published on: February 13, 2012
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Regulatory mechanisms controlling store-operated calcium entry
Goutham Kodakandla1, Askar M Akimzhanov2, Darren Boehning1
1Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA, 08103.
Arxiv
|September 25, 2023
Summary
Store-operated calcium entry (SOCE) is vital for cell function. This review details the molecular mechanisms, including STIM1 and Orai1 proteins, that regulate calcium influx and channel assembly.
Area of Science:
- Cellular Physiology
- Molecular Biology
- Ion Channel Function
Background:
- Calcium influx via plasma membrane ion channels is essential for cellular processes.
- Stimuli trigger inositol 1,4,5-trisphosphate (IP3) production, releasing calcium from the endoplasmic reticulum (ER).
- ER calcium depletion activates Stromal Interaction Molecule 1 (STIM1) and Orai1 channels, mediating store-operated calcium entry (SOCE).
Conclusions:
- SOCE is a fundamental mechanism regulated by STIM1 and Orai1.
- Understanding CRAC channel assembly into puncta remains an active area of research.
- This review provides a comprehensive overview of SOCE mechanisms and ongoing questions.
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