Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of superlattice phases and interparticle distance in the magnetic behaviour of SPION thin films.

Nanoscale·2025
Same author

Thermo-Convective Solution Growth of Vertically Aligned Zinc Oxide Nanowire Arrays for Piezoelectric Energy Harvesting.

Micromachines·2024
Same author

Understanding the Role of Solvent on the Growth of Zinc Oxide: Insight from Experiment and Molecular Dynamics Simulations.

Langmuir : the ACS journal of surfaces and colloids·2024
Same author

Monitoring the Morphological Changes of Skeleton-PtCo Electrocatalyst during PEMFC Start-Up/Shut-Down probed by in situ WAXS and SAXS.

ChemSusChem·2024
Same author

Positional control of DNA origami based gold dimer hybrid nanostructures on pre-structured surfaces.

Nanotechnology·2023
Same author

Conducting ITO Nanoparticle-Based Aerogels-Nonaqueous One-Pot Synthesis vs. Particle Assembly Routes.

Gels (Basel, Switzerland)·2023

Related Experiment Video

Updated: Jul 15, 2025

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately
08:39

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

Published on: July 8, 2014

25.2K

Smart Iterative Analysis Tool for the Size Distribution of Spherical Nanoparticles.

Jannik Guckel1,2, Marion Görke3, Georg Garnweitner2,3

  • 1Physikalisch-Technische Bundesanstalt, Bundesallee 100, Braunschweig 38116, Germany.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|September 25, 2023
PubMed
Summary

Accurate nanoparticle size analysis is crucial for performance. This study presents novel software using the circular Hough transform (CHT) for automated, precise nanoparticle detection, overcoming challenges like particle overlap and deformation.

Keywords:
OpenCVcircular Hough transformnanoparticleparticle size distribution analysispythontransmission electron microscopy

More Related Videos

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
09:33

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Published on: September 11, 2020

6.2K
Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles
11:54

Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles

Published on: June 25, 2018

10.4K

Related Experiment Videos

Last Updated: Jul 15, 2025

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately
08:39

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

Published on: July 8, 2014

25.2K
Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
09:33

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Published on: September 11, 2020

6.2K
Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles
11:54

Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles

Published on: June 25, 2018

10.4K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Image Analysis

Background:

  • Nanoparticle size is critical for performance, necessitating precise size distribution measurements.
  • Manual analysis of electron microscopy (EM) images for nanoparticle size is laborious and time-consuming.
  • Automated methods for nanoparticle detection in EM images are highly desired.

Purpose of the Study:

  • To introduce a novel automatic particle analysis software package.
  • To enhance the precision and efficiency of nanoparticle size distribution analysis.
  • To address limitations of existing methods, particularly concerning overlapping and deformed particles.

Main Methods:

  • Development of a software package utilizing the circular Hough transform (CHT) algorithm.
  • Implementation of an iterative workflow for CHT to ensure optimal detection across various particle radii.
  • Integration of smart intensity criteria to minimize false particle detection in challenging cases.

Main Results:

  • The software package demonstrated reliable particle analysis, effectively resolving common issues like particle overlap and deformation.
  • The iterative CHT workflow provided optimal detection over wide radius intervals.
  • Smart intensity criteria significantly reduced false positives, enhancing analysis precision.

Conclusions:

  • The developed software offers a reliable and automated solution for nanoparticle size analysis from EM images.
  • The novel approach effectively handles particle overlaps and deformations, improving accuracy and efficiency.
  • This tool addresses the long-standing need for precise, automated nanoparticle characterization.