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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Updated: Jul 15, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Functional Nucleic Acid Probes Based on Two-Photon for Biosensing.

Kefeng Wu1,2, Changbei Ma3, Yisen Wang1,2

  • 1GBA Branch of Aerospace Information Research Institute, Chinese Academy of Sciences, Guangzhou 510700, China.

Biosensors
|September 27, 2023
PubMed
Summary
This summary is machine-generated.

Functional nucleic acid (FNA) probes offer advantages for detection. Two-photon (TP) excitation enhances FNA probes, improving biomedical sensing with reduced photodamage and better tissue penetration compared to one-photon methods.

Keywords:
DNA nanotechnologyRNAbioimagingbiomoleculefluorescent probelight-activatedtwo-photon

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Area of Science:

  • Biomedical sensing and imaging
  • Molecular probes and diagnostics

Background:

  • Functional nucleic acid (FNA) probes are versatile tools in environmental, food, clinical, and imaging applications.
  • Conventional one-photon (OP) FNA probes face limitations like photodamage and poor light penetration in biological tissues.
  • Near-infrared (NIR) light-based two-photon (TP) excitation offers advantages over OP excitation for FNA probes.

Purpose of the Study:

  • To review recent advancements in two-photon (TP)-excited and -activated functional nucleic acid (FNA) probes.
  • To detail the applications of TP-based FNA probes in biomolecular detection.
  • To discuss the highlights, limitations, and design strategies for high-performance TP-based FNA probes.

Main Methods:

  • Review of literature on TP-excited and -activated FNA probes.
  • Analysis of TP probe properties: lower tissue absorption/autofluorescence, reduced photodamage/photobleaching, and higher spatial resolution.
  • Comparison of TP-based FNA probes with conventional OP-based FNA probes in biomedical sensing.

Main Results:

  • TP-based FNA probes exhibit superior performance in biomedical sensing due to enhanced optical properties.
  • TP excitation enables deeper tissue penetration and reduced phototoxicity, crucial for in vivo applications.
  • TP-based FNA probes offer improved spatial resolution and signal-to-noise ratios for biomolecular detection.

Conclusions:

  • TP-excited and -activated FNA probes represent a significant advancement over OP-based probes for biomedical sensing.
  • The unique properties of TP excitation facilitate more effective and less invasive biomolecular detection.
  • Further development of TP-based FNA probes is essential for expanding their biological and clinical applications.