Vancomycin-Stabilized Platinum Nanoparticles with Oxidase-like Activity for Sensitive Dopamine Detection

Area of Science:

• Nanomaterials Science
• Biosensors
• Analytical Chemistry

Background:

• Developing sensitive and reliable dopamine detection methods is crucial.
• Biosynthetic nanoparticles offer potential for novel biosensing applications.

Purpose of the Study:

• To synthesize and characterize vancomycin-stabilized platinum nanoparticles (Van-Pt_n NPs).
• To investigate the catalytic activity and stability of Van-Pt₂ NPs.
• To establish a colorimetric method for dopamine detection in human serum.

Main Methods:

• Vancomycin-stabilized platinum nanoparticles (Van-Pt_n NPs) were prepared using a biological template method.
• Oxidase-like and peroxidase-like activities of Van-Pt₂ NPs were evaluated.
• Stability (temperature, storage, salt solution) and cytotoxicity (A549 cells) were assessed.
• Colorimetric detection of dopamine (DA) in human serum was performed.

Main Results:

• Van-Pt₂ NPs demonstrated significant oxidase-like and peroxidase-like activities, attributed to reactive oxygen species generation.
• Van-Pt₂ NPs exhibited excellent stability and minimal cytotoxicity.
• A linear detection range of 10-700 μM for DA was achieved with a detection limit of 0.854 μM.

Conclusions:

• Van-Pt₂ NPs serve as effective nanozymes for dopamine detection.
• This study presents a rapid, reliable, and sensitive colorimetric method for dopamine analysis in biological samples.
• The findings highlight the utility of biosynthetic nanoparticles in advanced biosensing platforms.