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Insecticidal Effects of Receptor-Interference Isolated Bioactive Peptides on Fire Ant Colonies.

Satya Chinta^1,2, Robert Vander Meer¹, Erin O'Reilly¹

¹Center for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, Gainesville, FL 32608, USA.

International Journal of Molecular Sciences

|September 28, 2023

Summary

Novel Receptor-i technology identified potent bioactive peptides that effectively control red imported fire ant colonies. These peptides significantly reduced worker and queen survival, demonstrating a new pest control strategy.

Keywords:

GPCR Receptor-i bioactive peptides fire ant insecticide discovery neuropeptide

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Area of Science:

Entomology
Biochemistry
Pest Management

Background:

G-protein-coupled receptors (GPCRs) are crucial drug targets.
Receptor-interference (Receptor-i) is a novel technology for identifying receptor-specific bioactive peptides.
The red imported fire ant (Solenopsis invicta) poses a significant agricultural and medical threat.

Purpose of the Study:

To utilize Receptor-i technology to identify novel pest control agents targeting the fire ant pheromone biosynthesis activating neuropeptide receptor (PBAN-R).
To evaluate the efficacy of identified bioactive peptides against field colonies of Solenopsis invicta.

Main Methods:

Selected four cyclized bioactive peptides based on previous studies.
Conducted small-scale feeding bioassays to assess ant worker mortality.
Performed large-scale feeding trials on established fire ant colonies using the most effective peptides.

Main Results:

Two peptides, CMARYMSAC (MARY) and CIQQGSHFC (IQQG), showed significant efficacy.
MARY and IQQG treatments resulted in over 80% and 70% worker mortality, respectively, by day 84.
Treated queens experienced significant weight loss, and colony reproduction was impacted, unlike control colonies.

Conclusions:

Receptor-i technology successfully identified potent bioactive peptides for fire ant control.
The identified peptides, particularly MARY and IQQG, demonstrate significant potential as novel pest management agents.
This study provides proof-of-concept for Receptor-i and its applicability to other agricultural and medical pests.