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Updated: May 5, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Enhanced delignification and production of bioactive compounds in wheat straw by optimizing sterilization methods for
Peng Zhu1, Dongze Niu1, Sainan Zhang1
1Changzhou Key Laboratory of Biomass Green, Safe & High Value Utilization Technology, Institute of Urban and Rural Mining, Changzhou University, Changzhou 213164, China; National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.
Abstract:
This study aimed to examine the effects of sterilization methods on the degradation ability and bioactive compound production of Irpex lacteus in wheat straw. Following 28 days of fermentation, the lignin content of samples autoclaved and pasteurized at pH 4.5 was reduced by 16.0 % - 21.7 % compared to pasteurized samples without pH adjustment, accompanied by a significant increase in sugar yield ranging from 83.30 % - 96.35 %. Autoclaved samples exhibited the lowest total phenol content and antioxidant activity (P < 0.05). Bacillus occupied an absolute advantage (89.1 %) in samples pasteurized at pH 4.5, whereas 10 bacterial genera exhibited abundances above 1 % in pasteurized samples without pH adjustment. Furthermore, 45.1 % - 47.2 % of the metabolites comprised lipids and lipid-like molecules, and some of them were improved by pasteurization at pH 4.5. Overall, pasteurization at acidic conditions is an effective sterilization method for the fungal conversion of wheat straw.
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