Methylene Blue Assisted Electrochemical Detection of Bacterial Biofilm

This paper presents a novel electrochemical transduction method for the rapid and straightforward detection of bacterial biofilm. Briefly, fifteen isolates from various sources were collected and evaluated for their ability to generate biofilm. The Congo red-based agar method and the tube test were used for preliminary screening. A microtiter experiment was also performed to quantitatively examine the screening results and validate the outcomes of the proposed methylene blue-based electrochemical detection method. Electrochemical sensing was performed on the two selected isolates using methylene blue as a redox indicator. For optimization goals, several methylene blue concentrations were studied. Methylene blue at a concentration of 0.4 mM was used for the analysis conclusion. The developed electrochemical method displayed a linear R² value of 0.9747. The new electrochemical approach demonstrated great sensitivity and rapid response compared to conventional microtiter test methods.