Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

4.6K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
4.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

13
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
13
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.2K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-speed full-field swept-source dynamic optical coherence tomography enabled by neural-network-based image generation.

Biomedical optics express·2026
Same author

Dynamic full-field swept-source optical coherence tomography for high-resolution, long-depth, and intratissue-activity imaging.

Biomedical optics express·2026
Same author

<i>In vivo</i> dynamic optical coherence tomography of human skin with hardware- and software-based motion correction.

Biomedical optics express·2026
Same author

Dynamic optical coherence tomography algorithm for label-free assessment of swiftness and occupancy of intratissue moving scatterers.

Biomedical optics express·2026
Same author

Imaging of Tissue and Cell Dynamics: introduction to the feature issue.

Biomedical optics express·2026
Same author

Analysis of pulsatile motion in the murine cornea using phase-sensitive visible-light optical coherence tomography.

Biomedical optics express·2025
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Jul 15, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

4.9K

Multi-focus averaging for multiple scattering suppression in optical coherence tomography.

Lida Zhu1, Shuichi Makita1, Junya Tamaoki2

  • 1Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki, Japan.

Biomedical Optics Express
|October 4, 2023
PubMed
Summary
This summary is machine-generated.

Multi-focus averaging (MFA) enhances optical coherence tomography (OCT) imaging by reducing multiple scattering. This method improves image contrast in deep tissue regions, offering a cost-effective solution for better visualization.

More Related Videos

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.3K
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

7.7K

Related Experiment Videos

Last Updated: Jul 15, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

4.9K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.3K
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

7.7K

Area of Science:

  • Biomedical Optics
  • Medical Imaging

Background:

  • Multiple scattering in samples limits optical coherence tomography (OCT) penetration depth.
  • Reducing scattering is crucial for enhancing OCT image quality in deep tissues.

Purpose of the Study:

  • To introduce and validate the multi-focus averaging (MFA) method for suppressing multiple scattering in OCT.
  • To improve image contrast and penetration depth in scattering biological samples using OCT.

Main Methods:

  • The MFA method involves capturing multiple OCT volumes with varying focal positions.
  • Computational refocusing corrects defocus, followed by complex averaging of the OCT volumes.
  • Averaging suppresses multiply scattered light while preserving singly scattered light.

Main Results:

  • MFA significantly improved image contrast in both scattering phantoms and postmortem zebrafish compared to standard methods.
  • The method demonstrated superior contrast enhancement over single-focus averaging techniques.
  • MFA effectively reduced multiple-scattering signals, enhancing the single-scattering signal-to-noise ratio.

Conclusions:

  • Multi-focus averaging (MFA) is an effective technique for reducing multiple scattering in OCT imaging.
  • MFA offers a cost-effective approach to enhance contrast and image quality in deep tissue OCT.
  • The proposed method has significant implications for improving OCT-based tissue imaging and diagnostics.