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Updated: Jul 22, 2026

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
09:11

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination

Published on: September 25, 2014

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Evaluation of Latex Agglutination Test for Rapid Identification of Staphylococcus aureus Isolated from Pyogenic Wound

P Shah1, R Sah1, A Sharma1

  • 1Department of Microbiology B.P. Koirala Institute of Health Sciences (BPKIHS), Dharan, Sunsari, Nepal.

Kathmandu University Medical Journal (KUMJ)
|October 6, 2023
PubMed

Insights

The Latex agglutination test accurately identifies Staphylococcus aureus, including methicillin-resistant strains (MRSA). This rapid test offers high sensitivity and specificity, aiding in timely patient management.

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Diagnostic Testing

Background:

  • Staphylococcus aureus infections are a growing global health concern, leading to significant morbidity and mortality.
  • The emergence of multidrug-resistant strains, such as methicillin-resistant Staphylococcus aureus (MRSA), complicates treatment.
  • Rapid and accurate identification of Staphylococcus aureus is crucial for effective patient management.

Purpose of the Study:

  • To evaluate the performance of the Latex agglutination test (Staphaurex Plus) for identifying Staphylococcus aureus.
  • To compare the Latex agglutination test against the gold standard tube coagulase test.

Main Methods:

  • Standard microbiological methods were used for the isolation and identification of Staphylococci.
  • Methicillin-resistant Staphylococcus aureus (MRSA) detection followed Clinical and Laboratory Standard Institute guidelines.
  • The Staphaurex Plus Latex agglutination test was performed on 377 Staphylococci isolates according to manufacturer instructions.

Main Results:

  • The Latex agglutination test showed high positivity rates: 97.1% for methicillin-sensitive Staphylococcus aureus (MSSA) and 94.4% for MRSA.
  • Overall performance metrics included 95.46% sensitivity, 100% specificity, 100% positive predictive value, and 10.52% negative predictive value.
  • The test demonstrated excellent agreement with the tube coagulase test for Staphylococcus aureus identification.

Conclusions:

  • The Latex agglutination test (Staphaurex Plus) is a rapid and reliable method for identifying Staphylococcus aureus.
  • Its high accuracy supports its use in clinical settings for prompt diagnosis and management of Staphylococcus aureus infections.

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