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Decorated DNA-Based Scaffolds as Lateral Flow Biosensors
Simone Brannetti1, Serena Gentile1, Alejandro Chamorro-Garcia1
1Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy.
Angewandte Chemie (International Ed. in English)
|October 7, 2023
Summary
We developed novel DNA-based scaffolds for Lateral Flow Assays (LFAs). These engineered DNA structures enable sensitive and specific detection of diverse targets, from small molecules to proteins, using multiplexed platforms.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Biology
Background:
- Lateral Flow Assays (LFAs) are widely used for rapid diagnostics.
- There is a need for versatile and sensitive detection platforms for a broad range of analytes.
Purpose of the Study:
- To develop novel DNA-based scaffolds for enhanced Lateral Flow Assays (LFAs).
- To create functionalized DNA structures for multiplexed detection of various targets.
Main Methods:
- Re-engineering tile-based DNA tubular structures as functional scaffolds.
- Decorating DNA scaffolds with reporter tags, recognition elements (antigens, aptamers, proteins), and orthogonal fluorescent dyes.
- Developing sandwich and competitive multiplex lateral flow assay platforms.
Main Results:
- Demonstrated proof-of-principle for multiplexed LFAs using DNA scaffolds.
- Successfully detected small molecules (digoxigenin, dinitrophenol), antibodies, and proteins (thrombin).
- Achieved nanomolar sensitivity and high specificity in target detection.
Conclusions:
- Functional DNA-based scaffolds offer a versatile platform for LFAs.
- This approach combines DNA nanotechnology with LFA simplicity for broad target detection.
- The developed LFAs show potential for sensitive and specific analysis of diverse analytes.
