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Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
Published on: October 15, 2016
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[Single chain antibody fragment display systems: a review]
Yao Chen1,2, Xingfu Shu1,2, Yu Zhao1,2
1National Ethnic Affairs Commission Key Laboratory of Bioengineering and Technology, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, Gansu, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 8, 2023
Summary
Single chain antibody fragments (scFv) are versatile tools with advantages in molecular weight and expression. This review details scFv display systems for improved screening and applications in medicine and biology.
Area of Science:
- Biotechnology
- Immunology
- Molecular Biology
Background:
- Single chain antibody fragments (scFv) are the smallest functional antibody fragments, comprising variable heavy (VH) and variable light (VL) chains linked by a peptide.
- scFv offer advantages over intact antibodies, including smaller size, enhanced tissue penetration, reduced immunogenicity, and ease of expression.
- These fragments possess complete antigen-binding capabilities, making them valuable in various scientific fields.
Approach:
- This review systematically examines recent advancements in scFv display systems.
- Key systems discussed include phage, ribosome, mRNA, yeast cell surface, and mammalian cell display.
- The focus is on facilitating efficient scFv screening and broadening their applied research scope.
Key Points:
- scFv exhibit superior properties like low molecular weight, strong penetration, low immunogenicity, and straightforward expression.
- Diverse display systems (phage, ribosome, mRNA, yeast, mammalian) are crucial for scFv development.
- The review highlights the growing importance of scFv in medicine, biology, and food safety.
Conclusions:
- Advancements in scFv display systems are critical for efficient screening and application development.
- scFv technology holds significant promise for innovation in biotechnology and therapeutic development.
- Continued research into display systems will further unlock the potential of scFv across multiple disciplines.
