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Proteasome activity is required for reovirus entry into cells

Andrew T Abad1, Andrew J McNamara1, Pranav Danthi1

  • 1Department of Biology, Indiana University , Bloomington, Indiana, USA.

Journal of Virology
|October 13, 2023
PubMed
Abstract

Insights

Mammalian orthoreovirus (reovirus) replication depends on host systems. This study shows the proteasome system impacts reovirus entry and uncoating, revealing new host factors controlling viral replication.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Viruses require host cell machinery for replication due to limited genetic material.
  • Mammalian orthoreovirus (reovirus) serves as a model for studying host-virus interactions.

Purpose of the Study:

  • To investigate the role of the proteasome system in reovirus entry and replication.
  • To identify novel host factors influencing reovirus life cycle.

Main Methods:

  • Utilized chemical inhibitors to block proteasome function.
  • Observed the effects of proteasome inhibition on reovirus uncoating and replication.

Main Results:

  • Proteasome inhibition was found to block reovirus uncoating.
  • Inhibition of these processes led to reduced viral replication.

Conclusions:

  • The proteasome system is a critical host factor influencing reovirus entry and uncoating.
  • Additional host factors beyond the proteasome are involved in controlling reovirus replication.
Keywords:
entryreovirus

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