Related Experiment Video
Updated: Jul 13, 2025
12:24
Murine Model of CD40-activation of B cells
Published on: March 5, 2010
12.7K
Characterization of CD3+/CD20+ canine large-cell lymphoma
Cory M Howard1,2, Steffanie Anderson2, Bonnie Harrington3
1Department of Pathobiology and Diagnostic Investigation, Michigan State University College of Veterinary Medicine, East Lansing, MI, USA.
Summary
Canine large-cell lymphoma (LCL) with both T-cell (CD3) and B-cell (CD20) markers show T-cell receptor gamma rearrangements, similar to peripheral T-cell lymphoma (PTCL). This suggests a shared origin for these canine lymphoma types.
Area of Science:
- Veterinary Pathology
- Canine Oncology
- Molecular Diagnostics
Background:
- Immunophenotyping of canine large-cell lymphoma (LCL) aids in predicting clinical outcomes.
- CD3 expression typically indicates T-cell origin, while CD20 marks B-cells.
- A subset of canine LCLs co-expresses CD3 and CD20 (CD3+/CD20+), with poorly understood molecular characteristics.
Purpose of the Study:
- To investigate the immunophenotypic properties and antigen receptor clonality of canine CD3+/CD20+ LCL.
- To compare these features with peripheral T-cell lymphoma (PTCL) and diffuse large B-cell lymphoma (DLBCL).
Main Methods:
- Retrospective analysis of formalin-fixed, paraffin-embedded tissues from 10 CD3+/CD20+ LCL cases and controls.
- Polymerase chain reaction for antigen receptor rearrangement (PARR) to detect T-cell receptor gamma (TCRγ) and immunoglobulin heavy chain (IgH) rearrangements.
- Immunohistochemistry for B-cell markers (PAX5), BCL-2, and proliferation marker (Ki67).
Main Results:
- All CD3+/CD20+ LCL cases exhibited monoclonal TCRγ rearrangements.
- Three cases showed monoclonal IgH rearrangements, suggesting cross-lineage events.
- CD3+/CD20+ LCL shared frequent TCRγ rearrangement with PTCL but less frequent IgH rearrangement compared to DLBCL.
- PAX5 expression was lower in CD3+/CD20+ LCL than in DLBCL; BCL-2 expression was consistent.
Conclusions:
- Canine CD3+/CD20+ LCL cases demonstrate molecular and immunophenotypic properties predominantly resembling PTCL.
- These findings suggest a similar histogenesis for canine CD3+/CD20+ LCL and PTCL.
Related Concept Videos
01:22T Cell Activation and Clonal Selection
774
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
774
01:18Lymphoid Cells and Tissues
1.2K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
1.2K