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Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
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Developing a Cell-Microcarrier Tissue-Engineered Product for Muscle Repair Using a Bioreactor System.

Ana Luísa Cartaxo1,2, Ana Fernandes-Platzgummer1,2, Carlos A V Rodrigues1,2

  • 1Department of Bioengineering and Institute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.

Tissue Engineering. Part C, Methods
|October 16, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for treating fecal incontinence by attaching skeletal-derived muscle cells to biodegradable microcarriers. This regenerative medicine approach enhances cell viability and offers a promising new treatment option.

Keywords:
CD56TIPS PLGA microcarriersfecal incontinencemyotube formationskeletal derived muscle cells

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Area of Science:

  • Regenerative Medicine
  • Biotechnology
  • Gastroenterology

Background:

  • Fecal incontinence significantly impacts patient quality of life and incurs substantial economic costs.
  • Current treatments for fecal incontinence lack long-term efficacy.
  • Regenerative medicine offers potential for sphincter muscle regeneration and restoring continence.

Purpose of the Study:

  • To develop and validate a novel bioprocessing method for delivering skeletal-derived muscle cells (SkMDCs) for fecal incontinence treatment.
  • To create a scalable, two-step process combining SkMDC expansion with microcarrier-based delivery.
  • To assess the viability and myogenic potential of SkMDCs attached to biodegradable microcarriers.

Main Methods:

  • Patient-isolated SkMDCs were expanded using static culture systems.
  • Expanded SkMDCs were combined with poly(lactic-co-glycolic acid) microcarriers in a Vertical-Wheel® bioreactor.
  • Cell attachment efficiency, viability, and myogenic marker expression (CD56) were analyzed.

Main Results:

  • The bioprocessing method achieved up to 80% efficiency in attaching SkMDCs to microcarriers.
  • SkMDCs remained viable throughout the process and retained myogenic features.
  • Cells cultured on microcarriers demonstrated migration and differentiation into myotubes, indicating regenerative potential.

Conclusions:

  • This study presents a proof-of-concept for an innovative SkMDC delivery system using biodegradable microcarriers.
  • The developed method shows promise for improving cell viability and facilitating sphincter muscle regeneration.
  • This approach could lead to a new, effective treatment strategy for fecal incontinence.