Response surface methodology use in construction of polianiline-coated carbon paste electrode-based biosensor:...

Area of Science:

• Electrochemistry
• Biosensors
• Biotechnology

Background:

• Amperometric biosensors are crucial for detecting various analytes.
• Optimizing biosensor construction and operating conditions is key to enhancing performance.
• Polyaniline (PANI) and glucose oxidase (GOD) are common components in glucose biosensors.

Purpose of the Study:

• To investigate the effects of biosensor construction and operating conditions on current response.
• To optimize amperometric biosensors for glucose, sucrose, and lactose detection.
• To enhance the performance of carbon paste electrodes (CPEs) using novel modifications.

Main Methods:

• Synthesized polyaniline (PANI) on carbon paste electrode (CPE) via cyclic voltammetry.
• Immobilized glucose oxidase (GOD) to construct the amperometric biosensor.
• Utilized Box-Behnken and optimal designs for optimizing preparation and operating conditions (pH, potential).
• Incorporated 2-cyanoethylpyrrole (CPy) to modify the CPE and enhance electron transfer.
• Constructed sucrose- and lactose-sensitive biosensors by co-immobilizing GOD with invertase (INV) or β-galactosidase.

Main Results:

• Sodium oxalate (NaO_x) concentration significantly affected current response during biosensor preparation.
• pH was a critical factor in optimizing operating conditions for current measurement.
• 2-cyanoethylpyrrole (CPy) modification substantially enhanced biosensor efficacy, likely by increasing electron transfer rate.
• CPy modification led to significant increases in I_max /K_M values for glucose (11.8x), sucrose (7.83x), and lactose (2.56x) biosensors.

Conclusions:

• Optimized experimental designs effectively improved amperometric biosensor performance.
• CPy modification is a promising strategy for developing highly sensitive and efficient biosensors.
• The developed biosensors demonstrate potential for accurate detection of glucose, sucrose, and lactose.