Catcher/Tag Toolbox: Biomolecular Click-Reactions For Protein Engineering Beyond Genetics
1Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P.O. Box 16100, 02150, Espoo, Finland.
Chembiochem : a European Journal of Chemical Biology
|October 18, 2023
Summary
Catcher/Tag systems offer precise protein conjugation via isopeptide bonds for diverse applications. Future developments aim to enhance control over this versatile protein modification technique.
Area of Science:
- Biochemistry
- Molecular Biology
- Synthetic Biology
Background:
- Protein conjugation beyond genetic methods is a growing area of interest.
- Catcher/Tag systems utilize isopeptide bonds for specific protein conjugation in vitro and in vivo.
- These systems offer efficient, robust, and irreversible protein modification strategies.
Purpose of the Study:
- To summarize recent advancements in the Catcher/Tag toolbox.
- To highlight the design of Catcher/Tag pairs for specific applications.
- To discuss limitations and future directions for Catcher/Tag systems.
Main Methods:
- Review of Catcher/Tag systems and their applications.
- Emphasis on the design principles of Catcher/Tag pairs.
- Analysis of current limitations, including pH sensitivity.
Main Results:
- Catcher/Tag systems are versatile tools for enzyme industry, vaccines, biomaterials, and cellular applications.
- Specific Catcher/Tag pairs have been designed for targeted uses.
- The pH sensitivity of these reactions is a key consideration.
Conclusions:
- Catcher/Tag systems represent a powerful method for protein conjugation.
- Further improvements in controlling ligation induction will expand their applications.
- This technology holds significant promise for future biotechnological innovations.
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