Related Experiment Video
Updated: Jul 12, 2025
06:18
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
1.4K
Vancomycin and Gentamicin Removal with the HA380 Cartridge during Experimental Hemoadsorption
Taku Furukawa1, Yugeesh Lankadeva2,3, Ian Charles Baldwin4
1Preclinical Critical Care Unit, The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, Victoria, Australia, taku.furukawa@florey.edu.au.
Blood Purification
|October 19, 2023
Summary
Hemoadsorption using the HA380 cartridge significantly removes vancomycin and gentamicin in sepsis patients. Clinicians should consider increasing antibiotic doses by 25-35% during this therapy.
Area of Science:
- Pharmacokinetics
- Critical Care Medicine
- Biomaterials Science
Background:
- Hemoadsorption is an emerging adjunctive therapy for sepsis.
- Its effect on antibiotic concentrations, specifically vancomycin and gentamicin, is not well understood.
- The HA380 cartridge, a novel styrene-divinylbenzene copolymer, is investigated for its removal capabilities.
Purpose of the Study:
- To evaluate the in vivo removal efficiency of vancomycin and gentamicin during hemoadsorption with the HA380 cartridge.
- To determine the impact of HA380 hemoadsorption on antibiotic levels in a sepsis model.
- To provide data for optimizing antibiotic dosing in patients undergoing hemoadsorption therapy.
Main Methods:
- An experimental study was conducted on six surgically prepared sheep.
- Sheep received vancomycin and gentamicin, followed by a continuous vancomycin infusion.
- Hemoadsorption was performed using the HA380 cartridge at a blood flow of 120 mL/min, with removal ratios and clearance calculated.
Main Results:
- The HA380 cartridge demonstrated high initial removal ratios for both vancomycin (>90%) and gentamicin (>96.9%).
- Over four hours, removal ratios declined, with vancomycin at 28.0% and gentamicin at 53.0%.
- Sorbent-based clearance was significantly lower for vancomycin compared to gentamicin (P < 0.0001).
Conclusions:
- The HA380 sorbent cartridge is safe and effectively removes vancomycin and gentamicin over a four-hour period.
- Significant antibiotic removal necessitates dose adjustments in patients undergoing HA380 hemoadsorption.
- An additional 25-35% antibiotic dose may be required during therapy to maintain therapeutic levels.
Related Concept Videos
01:15Dialysis
709
Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
709
01:24Hemodialysis II: Procedure and Complications
27
DialyzersA hemodialysis (HD) dialyzer is a plastic cartridge containing thousands of parallel hollow fibers, which serve as semipermeable membranes. These fibers are typically made from cellulose-based or other synthetic materials. During HD, blood is pumped into the top of the cartridge and distributed among these fibers. Simultaneously, dialysis fluid, known as dialysate, is introduced into the bottom of the cartridge, bathing the outside of the fibers. Across the semipermeable membrane,...
27