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BDNF-loaded PDADMAC-heparin multilayers: a novel approach for neuroblastoma cell study.
Maria Dąbkowska1, Iga Stukan2, Bogusław Kowalski3
1Independent Laboratory of Pharmacokinetic and Clinical Pharmacy, Pomeranian Medical University, Rybacka 1, 70-204, Szczecin, Poland. maria.dabkowska@pum.edu.pl.
Scientific Reports
|October 20, 2023
Summary
This study developed polyelectrolyte multilayers (PEMs) as a novel carrier for brain-derived growth factor (BDNF). These PEMs enhance BDNF delivery, exhibit antioxidant properties, and promote 3D cell structure formation for biomedical applications.
Area of Science:
- Biomaterial Science
- Nanotechnology
- Biomedical Engineering
Background:
- Biomaterial science advances nanoscale materials for drug delivery and therapeutic applications.
- Polyelectrolyte multilayers (PEMs) are versatile platforms for biomolecule immobilization.
- Brain-derived growth factor (BDNF) is crucial for neuronal development and function.
Purpose of the Study:
- To develop and characterize PEMs for efficient adsorption and delivery of BDNF.
- To investigate the in vitro effects of BDNF-loaded PEMs on neuroblastoma cells.
- To evaluate the antioxidant properties and 3D structure formation capabilities of the developed PEMs.
Main Methods:
- Sequential adsorption of polydiallyldimethylammonium chloride (PDADMAC) and heparin sodium salt (HP) to form PEMs.
- Characterization using electrokinetic (SPM) and optical (OWLS) techniques.
- In vitro studies using SH-SY5Y neuroblastoma cells, including ELISA, cell viability assays, and HPLC analysis.
Main Results:
- BDNF was significantly adsorbed onto HP-terminated PEMs under physiological conditions.
- PEMs enhanced BDNF cellular uptake and reduced cell viability and mitochondrial membrane polarization.
- PEMs demonstrated significant antioxidative properties, reducing lipid peroxidation by nearly 50%.
- Enhanced formation of spheroid-like, 3D cellular structures was observed.
Conclusions:
- Developed PEMs effectively carry and stabilize BDNF, enhancing its accessibility to cells.
- PEMs possess inherent antioxidant properties beneficial for cellular environments.
- These PEMs show promise for future biomedical applications, including anticancer adjuvant therapy and neurodegenerative disease modeling.
