Related Experiment Video
Updated: Jul 12, 2025
Tracking Mouse Bone Marrow Monocytes In Vivo
Published on: February 27, 2015
Protocol for live imaging of transferred mouse bone marrow cells by two-photon microscopy
Takao Sudo1, Erika Yamashita1, Junichi Kikuta1
1Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
Abstract:
The in situ behavior of living cells can be visualized by two-photon microscopy. Here, we present a protocol for the live imaging of transferred mouse bone marrow cells by two-photon microscopy. We describe steps for staining and injecting target cells into mice, fixing the skull bone to a head holder and stage, and 4D imaging bone marrow using multi-photon microscopy. We then detail procedures for creating images and analyzing cells. For complete details on the use and execution of this protocol, please refer to Sudo et al. (2021).1.
