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Updated: Jul 12, 2025
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Tracking Mouse Bone Marrow Monocytes In Vivo
Published on: February 27, 2015
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Protocol for live imaging of transferred mouse bone marrow cells by two-photon microscopy
Takao Sudo1, Erika Yamashita1, Junichi Kikuta1
1Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
STAR Protocols
|October 21, 2023
Summary
This study details a two-photon microscopy protocol for live imaging mouse bone marrow cells. The method enables visualization of in situ cellular behavior for research applications.
Area of Science:
- Cellular Biology
- Microscopy Techniques
- Immunology
Background:
- Visualizing live cell behavior in situ is crucial for understanding biological processes.
- Two-photon microscopy offers deep tissue penetration and reduced phototoxicity for live imaging.
Purpose of the Study:
- To present a detailed protocol for live imaging of transferred mouse bone marrow cells using two-photon microscopy.
- To enable 4D imaging and analysis of bone marrow cells in situ.
Main Methods:
- Protocol development for staining and injecting mouse bone marrow cells.
- Skull bone fixation for stable head holding and stage mounting.
- 4D imaging of bone marrow using multi-photon microscopy.
- Image creation and cell analysis procedures.
Main Results:
- Successful live imaging of transferred mouse bone marrow cells.
- Demonstration of 4D imaging capabilities for bone marrow analysis.
- Detailed procedures for image acquisition and data analysis.
Conclusions:
- The presented protocol provides a robust method for live imaging of mouse bone marrow cells.
- This technique facilitates in situ studies of cellular dynamics within the bone marrow niche.
- The protocol supports advanced research in bone marrow biology and related fields.
