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Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma

M Li1, J An2, H Ren3

  • 1Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Bulletin of Experimental Biology and Medicine
|October 24, 2023
PubMed

Insights

Colon cancer-associated transcript 2 (CCAT2), a long noncoding RNA, is highly expressed in laryngeal squamous cell carcinoma (LSCC). Silencing CCAT2 inhibits LSCC cell proliferation and invasion, suggesting it as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Laryngeal squamous cell carcinoma (LSCC) is a significant health concern.
  • The role of long noncoding RNAs (lncRNAs) in LSCC pathogenesis is increasingly recognized.
  • Colon cancer-associated transcript 2 (CCAT2) is a lncRNA implicated in various cancers.

Purpose of the Study:

  • To investigate the biological role and underlying mechanism of CCAT2 in human LSCC.
  • To determine the correlation between CCAT2 expression and LSCC patient prognosis.
  • To evaluate CCAT2 as a potential therapeutic target and prognostic biomarker for LSCC.

Main Methods:

  • Quantitative real-time PCR to measure CCAT2 expression in LSCC tissues and cell lines.
  • Cell Counting Kit-8, Transwell assay, and flow cytometry to assess cell proliferation, invasion, and apoptosis.
  • In vivo LSCC xenograft model in nude mice to evaluate tumor growth.
  • Western blotting and immunohistochemistry to detect target protein expression (β-catenin, CDK8).

Main Results:

  • CCAT2 expression was significantly elevated in LSCC tissues and cells compared to normal controls.
  • High CCAT2 expression correlated with poorer 5-year overall survival in LSCC patients.
  • CCAT2 knockdown suppressed LSCC cell proliferation and invasion while promoting apoptosis.
  • In vivo, CCAT2 knockdown inhibited tumor growth, volume, and weight.
  • CCAT2 silencing down-regulated β-catenin and CDK8 expression, inhibiting the Wnt/β-catenin signaling pathway.

Conclusions:

  • CCAT2 plays a crucial role in promoting LSCC progression by enhancing proliferation and invasion.
  • CCAT2 acts, at least in part, by modulating the Wnt/β-catenin signaling pathway.
  • CCAT2 is a potential prognostic biomarker and therapeutic target for LSCC.
Keywords:
apoptosiscyclin-dependent kinase 8laryngeal squamous cell carcinomalong noncoding RNA colon cancer-associated transcript 2 (CCAT2)proliferation

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