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Updated: Jul 12, 2025

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers
Published on: September 4, 2015
Protocol for potential energy-based bifurcation analysis, parameter searching, and phase diagram analysis of
Debashis Barik1, Soutrick Das1
1School of Chemistry, University of Hyderabad, Central University P.O., Hyderabad, Telangana 500046, India.
Abstract:
We have explored the design principles of noncanonical bistable switches using high-throughput bifurcation analysis of positive feedback loops under dual signaling. Here, we present a protocol to carry out bifurcation analysis using pseudo-potential energy of the dynamical system. We also describe steps to perform automated parameter searching for canonical and noncanonical switches and multi-parameter phase diagram analysis of these switches. For complete details on the use and execution of this protocol, please refer to Das et al.1.
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