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Methods to assess radiation-induced cardiotoxicity in rodent models
Anupama Melam1, Lauren N Pedersen1, Amanda Klaas1
1Department of Radiation Oncology, Washington University in St. Louis, St. Louis, MO, United States.
Methods in Cell Biology
|October 27, 2023
Summary
Cancer survivors receiving thoracic radiation therapy face risks of heart damage (radiation-induced cardiotoxicity). This chapter guides researchers on using animal models to study and prevent this critical side effect.
Area of Science:
- Oncology
- Cardiology
- Radiation Oncology
Background:
- Thoracic radiation therapy can lead to radiation-induced cardiotoxicity (RICT) in cancer survivors.
- Advancements in radiation delivery have reduced but not eliminated cardiac exposure.
- RICT poses long-term morbidity and mortality risks, with current inability to predict susceptible individuals.
Purpose of the Study:
- To provide foundational and technical knowledge for using rodent models in RICT research.
- To guide researchers in preclinical investigations for better RICT prevention, detection, and management.
Main Methods:
- Overview of RICT pathophysiology and clinical manifestations.
- Discussion of RICT study design considerations, including animal selection and radiation planning.
- Example protocols for murine tissue harvesting and processing for downstream applications.
Main Results:
- N/A (Chapter provides methodology and guidance, not experimental results).
Conclusions:
- Pre-clinical investigations using animal models are crucial for understanding and mitigating RICT.
- Standardized methodologies are needed to advance RICT research and improve cancer survivor outcomes.
