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Published on: June 29, 2014
Disinfection, sterilization and antisepsis: An overview.
William A Rutala1, John M Boyce2, David J Weber3
1Statewide Program for Infection Control and Epidemiology, UNC School of Medicine, Chapel Hill, NC; Division of Infectious Diseases, UNC School of Medicine, Chapel Hill, NC.
Proper disinfection and sterilization of medical devices are crucial for preventing infections during surgical procedures. Adhering to guidelines ensures patient safety and reduces exposure to infectious agents.
Area of Science:
- Infection Prevention and Control
- Medical Device Sterilization
- Public Health
Background:
- Millions of surgical procedures occur annually, posing infection risks.
- Medical devices contact sterile tissues and mucous membranes.
- Preventing infection is paramount in healthcare settings.
Purpose of the Study:
- To review the literature on disinfectants, sterilization, and antiseptics.
- To outline appropriate disinfection and sterilization levels for medical devices.
- To emphasize the importance of infection control in surgical procedures.
Main Methods:
- Systematic literature search for disinfection and sterilization methods.
- Analysis of effectiveness of disinfectants and antiseptics.
- Categorization of medical items based on contact risk.
Main Results:
- Disinfection levels (high, low) depend on item classification (critical, semicritical, noncritical).
- Cleaning is a mandatory precursor to high-level disinfection and sterilization.
- Antiseptics are vital for hand hygiene, surgical antisepsis, and skin preparation.
Conclusions:
- Effective disinfection and sterilization ensure safe medical device use.
- Strict adherence to guidelines prevents patient infections.
- Proper protocols minimize exposure to infectious agents.
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Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Asepsis
Hand hygiene
Hand washing...
Methods for Controlling Microbial Growth
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...

