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MicroRNA Expression Profile in Bone Marrow and Lymph Nodes in B-Cell Lymphomas
Yuliya A Veryaskina1,2, Sergei E Titov1,3, Igor B Kovynev4
1Department of the Structure and Function of Chromosomes, Laboratory of Molecular Genetics, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.
International Journal of Molecular Sciences
|October 28, 2023
Summary
MicroRNA (miRNA) expression profiles differ between bone marrow and lymph nodes in various B-cell lymphomas. This finding aids in differentiating lymphoma types and understanding lymphopoiesis impairment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Hodgkin's lymphoma (HL) and non-Hodgkin's lymphomas (NHL) originate from distinct B-cell differentiation stages.
- MicroRNA (miRNA) expression patterns are dynamic during lymphopoiesis, suggesting their potential as diagnostic markers.
- Understanding miRNA dysregulation in lymphopoiesis is crucial for fundamental research and clinical applications.
Purpose of the Study:
- To investigate and compare unique miRNA expression profiles across different types of B-cell lymphomas.
- To identify specific miRNA signatures that can differentiate between various lymphoma subtypes.
- To explore the role of miRNA expression differences in lymph nodes versus bone marrow within lymphoma contexts.
Main Methods:
- Analysis of specific miRNA expression levels (miRNA-18a, -20a, -96, -182, -183, -26b, -34a, -148b, -9, -150, -451a, -23b, -141, and -128).
- Utilized real-time RT-PCR for quantitative analysis.
- Examined miRNA expression in lymph node (LN) and bone marrow (BM) samples from patients with HL, diffuse large B-cell lymphoma (DLBCL), mantle cell lymphoma (MCL), follicular lymphoma (FL), lymphadenopathy (LA), and non-cancerous blood diseases (NCBD).
Main Results:
- Observed significant alterations in BM miRNA levels: increased expression of miRNA-26b, -150, and -141 in MCL; decreased expression of the miR-183-96-182 cluster and miRNA-451a in DLBCL, compared to NCBD.
- Detected distinct LN miRNA profiles: increased miR-183-96-182 cluster in MCL, and miRNA-18a, miRNA-96, and miRNA-9 in FL.
- Found decreased LN expression of miRNA-150 in DLBCL, and miRNA-182, miRNA-150, and miRNA-128 in HL, relative to controls.
Conclusions:
- Distinct miRNA expression profiles exist between bone marrow and lymph nodes, varying by B-cell lymphoma type.
- These observed miRNA signature differences may be influenced by the tumor microenvironment.
- miRNA expression analysis holds potential as a diagnostic tool for differentiating B-cell lymphomas.
