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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Jul 12, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

High-throughput multiplexed fluorescence lifetime microscopy.

Zhimin Zhang, Xin Liu, Minfei He

|November 1, 2023

Summary

This summary is machine-generated.

A new method enhances high-throughput multiplexed fluorescence lifetime imaging using a computer-generated hologram and advanced photon counting. This breakthrough increases data collection speed fivefold, enabling simultaneous dual-target measurements for cellular and tissue analysis.

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Last Updated: Jul 12, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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Published on: February 9, 2012

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

Area of Science:

Biophotonics
Microscopy
Optical Imaging

Background:

Fluorescence lifetime microscopy (FLIM) is crucial for cellular interaction analysis and histopathological identification.
Current FLIM techniques face limitations in throughput and multiplexing capabilities.

Purpose of the Study:

To develop a novel high-throughput multiplexed fluorescence lifetime imaging system.
To enhance the speed and efficiency of fluorescence lifetime data acquisition.

Main Methods:

Generated a uniformed illumination optical focus array using a computer-generated hologram algorithm based on matrix triple product.
Employed an array detector and multichannel time-correlated single-photon counting (TCSPC).
Utilized interval segmentation of photon time detection for high-throughput acquisition.

Main Results:

Achieved a fivefold increase in fluorescence lifetime collection throughput.
Demonstrated capability for simultaneous dual-target fluorescence lifetime measurement.
Validated the effectiveness of the novel computer-generated hologram and TCSPC approach.

Conclusions:

The presented method significantly advances high-throughput multiplexed fluorescence lifetime imaging.
This technique offers enhanced capabilities for biological and medical imaging applications.
The system provides a faster and more efficient approach to FLIM data acquisition.