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Published on: October 23, 2011
Detection of Trichomonas vaginalis by Allplex™ STI Essential Assay (Seegene) in clinical samples from the Brazilian
Mariana Dicki Freitas1, Fernanda Gomes Cardoso1, Graziela Vargas Rigo1
1Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, Porto Alegre, Rio Grande do Sul, 90610-000, Brazil.
Abstract:
The failures in Trichomonas vaginalis (TV) infection diagnosis leave more than half of cases unidentified. In this report, urine and vaginal discharge samples were analyzed by wet mount, culture examination, and real-time PCR by Allplex™ (Seegene®) kit, in a population assisted by the Brazilian Public Health System. From 747 samples, 2.81% were positive for TV in wet mount and culture, and 3.88% by Allplex™. Samples kept at - 80 ºC for 22 months did not impair the PCR technique. The sensitivity for wet mount, culture, and Allplex™ was 72, 100, and 100%, respectively. Allplex™ technique showed highest detection of TV.
