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Published on: August 10, 2017
Components Subcellular Localization: Identification of Lipoproteins Using Globomycin and Radioactive Palmitate
1Institut Pasteur, Université Paris Cité, CNRS UMR6047, INSERM U1306, Biology and genetics of the bacterial cell wall Unit, 25-28 rue du docteur Roux, Paris cedex 15, France. nienke.buddelmeijer@pasteur.fr.
Abstract:
Bacterial lipoproteins are characterized by fatty acids, derived from membrane phospholipids, which are covalently attached to their amino terminus via posttranslational modification in the cytoplasmic membrane. Here, I describe the detection of one of the intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using 3H-palmitate labeling and inhibition of signal peptidase II (Lsp) by globomycin and detection by fluorography.
