Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.2K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.4K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.4K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.3K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

9.2K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitigation of artifacts in imaging biosamples with optical scanning transmission electron microscopy.

Applied microscopy·2026
Same author

Viral transcriptional regulators extensively rewire host pathways through diverse mechanisms.

bioRxiv : the preprint server for biology·2026
Same author

Raman-based label-free microscopic analysis of the pancreas in living zebrafish larvae.

FEBS open bio·2025
Same author

Workflow for Fluorescence-Targeted Lamella Milling From Vitrified Cells With a Coincident Fluorescence, Electron, and Ion Beam Microscope.

Bio-protocol·2025
Same author

Automated analysis of ultrastructure through large-scale hyperspectral electron microscopy.

Npj imaging..·2025
Same author

Pancreatic exocrine damage induces beta cell stress in zebrafish larvae.

Diabetologia·2025
Same journal

Deep PACBED: Multitask analysis of PACBED images using deep neural networks.

Ultramicroscopy·2026
Same journal

Guided progressive reconstructive imaging: A new quantization-based framework for low-dose, high-throughput and real-time analytical ptychography.

Ultramicroscopy·2026
Same journal

Brightness optimization in a 200 keV DTEM source by geometry-driven aberration suppression.

Ultramicroscopy·2026
Same journal

Characterization of the Timepix4 hybrid pixel detector and its impact on four-dimensional scanning transmission electron microscopy (4D-STEM).

Ultramicroscopy·2026
Same journal

Contamination analysis of the residual gas composition in transmission electron microscopy.

Ultramicroscopy·2026
Same journal

Temperature-dependent mean inner potential of polystyrene spheres measured using off-axis electron holography.

Ultramicroscopy·2026
See all related articles

Related Experiment Video

Updated: Jul 11, 2025

Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.1K

Optical STEM detection for scanning electron microscopy.

Arent J Kievits1, B H Peter Duinkerken2, Job Fermie3

  • 1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands.

Ultramicroscopy
|November 6, 2023
PubMed
Summary
This summary is machine-generated.

Optical scanning transmission electron microscopy (OSTEM) offers a novel, faster detection method for electron microscopy. This technique achieves high-resolution imaging comparable to existing methods, potentially increasing throughput for biological tissue analysis.

Keywords:
Electron detectionInstrumentation developmentScanning electron microscopyScanning transmission electron microscopyVolume electron microscopy

More Related Videos

Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

7.4K
Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope
11:14

Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope

Published on: May 28, 2016

13.9K

Related Experiment Videos

Last Updated: Jul 11, 2025

Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.1K
Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

7.4K
Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope
11:14

Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope

Published on: May 28, 2016

13.9K

Area of Science:

  • Electron Microscopy
  • Biological Imaging
  • Materials Science

Background:

  • Advances in electron microscopy enable large-scale volumetric imaging of biological tissues.
  • Current electron microscope throughput is limited by detection speed, hindering high-resolution imaging of large volumes.
  • Developing faster detection methods is crucial for improving imaging throughput.

Purpose of the Study:

  • To characterize and benchmark optical scanning transmission electron microscopy (OSTEM) as a novel detection technique.
  • To compare OSTEM performance against established detection methods in scanning electron microscopy.

Main Methods:

  • Qualitative and quantitative comparison of OSTEM with secondary electron (SE), backscattered electron (BSE), and annular dark-field (ADF) detection.
  • Benchmarking performed in scanning transmission electron microscopy (STEM) mode.

Main Results:

  • OSTEM produces images with contrast, resolution, and signal-to-noise ratio comparable to BSE detection.
  • OSTEM demonstrates potential for enhancing throughput in large-scale imaging applications.
  • The technique shows promise for accelerating imaging in single-beam scanning electron microscopes.

Conclusions:

  • OSTEM is a viable and effective detection technique for scanning electron microscopy.
  • It complements existing methods for large-scale imaging, particularly in (scanning) transmission electron microscopy.
  • OSTEM has the potential to significantly increase imaging speed and efficiency.