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Engineering status of protein for improving microbial cell factories
Pei Zhou1, Cong Gao1, Wei Song2
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Biotechnology Advances
|November 8, 2023
Summary
Engineering protein status in microbial cell factories (MCFs) enhances bioproduction efficiency. This review details strategies like protein engineering and modification to boost catalytic and metabolic capacities for sustainable chemical synthesis.
Area of Science:
- Metabolic Engineering
- Synthetic Biology
- Biotechnology
Background:
- Microbial cell factories (MCFs) offer sustainable chemical synthesis from renewable resources.
- Protein status significantly impacts MCF efficiency, often limiting production titers, yields, and productivity.
- Optimizing protein status is crucial for advancing bioproduction.
Purpose of the Study:
- To review engineering strategies for optimizing metabolic protein status in MCFs.
- To provide insights into enhancing microbial catalytic, metabolic, and synthetic capacities.
- To highlight future directions for improving MCFs through protein status engineering.
Main Methods:
- Summarizing protein engineering approaches to boost microbial catalytic efficiency.
- Reviewing protein modification techniques for regulating metabolic capacity.
- Discussing protein assembly strategies for enhancing synthetic capacity.
Main Results:
- Protein engineering can significantly improve enzyme activity and specificity.
- Protein modification allows fine-tuning of metabolic pathways and flux.
- Protein assembly enhances the efficiency of multi-step biosynthetic pathways.
Conclusions:
- Engineering protein status is a key strategy for developing high-performance MCFs.
- Further research into protein engineering, modification, and assembly will drive innovation in bioproduction.
- Optimized protein status promises more efficient and sustainable chemical manufacturing.
Keywords:
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