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Measurement of Ice Nucleation Activity of Biological Samples
Rosemary J Eufemio1, Ralph Schwidetzky2, Konrad Meister3,4,5
1Biomolecular Sciences Graduate Program, Boise State University, Boise, ID, USA.
Abstract:
Experimentation with ice-nucleating biomolecules is needed to advance the fundamental understanding of biotic heterogeneous ice nucleation. Standard experimental procedures vary with sample type. Here we describe a generalized primary purification and analysis process to measure ice nucleation activity of biological samples using an advanced freezing droplet assay.
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