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Related Concept Videos

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Related Experiment Video

Updated: Jul 11, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
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Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy in an Integrated Setup.

Julius Reinhard1,2, Sophia Kaleta1, Johann Jakob Abel1

  • 1Institute of Optics and Quantum Electronics, Friedrich Schiller University Jena, Max-Wien-Platz 107743 Jena, Germany.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|November 9, 2023
PubMed
Summary
This summary is machine-generated.

A new compact table-top soft X-ray microscope enables correlative imaging by combining fluorescence and X-ray microscopy. This technique achieves nanoscale structural resolution with functional contrast for biological samples without altering them.

Keywords:
cell imagingcorrelative microscopyfluorescence microscopylaboratory-basedlaser-produced plasmawater window X-ray microscopyzone plates

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Area of Science:

  • Microscopy
  • Biophysics
  • Nanotechnology

Background:

  • Correlative microscopy integrates multiple imaging techniques for comprehensive sample analysis.
  • Fluorescence microscopy offers functional contrast but limited structural detail.
  • Soft X-ray microscopy provides label-free structural contrast but requires complex setups.

Purpose of the Study:

  • To develop a compact, laboratory-based soft X-ray microscope for correlative imaging.
  • To combine the strengths of fluorescence and soft X-ray microscopy.
  • To enable in situ correlative imaging without sample manipulation.

Main Methods:

  • Development of a table-top soft X-ray microscope with integrated epifluorescence.
  • Utilizing laser-produced plasmas for sufficient soft X-ray photon flux.
  • Performing correlative imaging on various samples, including cells and nanoparticles.

Main Results:

  • Achieved nanoscale structural resolution down to 50 nm.
  • Demonstrated successful multimodal imaging of nanoparticles and multicolor labeled cells.
  • Ensured identical measurement conditions by keeping samples in place.

Conclusions:

  • The developed compact soft X-ray microscope facilitates in situ correlative imaging.
  • This approach merges nanoscale structural resolution with functional contrast.
  • The system offers a powerful tool for investigating complex biological and nanomaterials.