Related Experiment Video
Updated: Jul 11, 2025
A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
Published on: May 10, 2022
A pipeline for STED super-resolution imaging and Imaris analysis of nanoscale synapse organization in mouse cortical
Ezra Kruzich1, Rhushikesh A Phadke2, Alison Brack2
1Neurobiology Section in the Department of Biology, Boston University, Boston, MA 02215, USA.
Abstract:
Advances in super-resolution imaging enable us to delve into its intricate structural and functional complexities with unprecedented detail. Here, we present a pipeline to visualize and analyze the nanoscale organization of cortical layer 1 apical dendritic spines in the mouse prefrontal cortex. We describe steps for brain slice preparation, immunostaining, stimulated emission depletion super-resolution microscopy, and data analysis using the Imaris software package. This protocol allows the study of physiologically relevant brain circuits implicated in neuropsychiatric disorders.
More Related Videos
08:49Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
Published on: August 1, 2022
09:49Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
Published on: October 8, 2013