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Updated: Jun 13, 2026

Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
Multiplexed Digital Characterization of Misfolded Protein Oligomers via Solid-State Nanopores
Sarah E Sandler1, Robert I Horne2, Sara Rocchetti1
1Cavendish Laboratory, Maxwell Centre, Department of Physics, University of Cambridge, Cambridge CB3 0HE, U.K.
Abstract:
Misfolded protein oligomers are of central importance in both the diagnosis and treatment of Alzheimer's and Parkinson's diseases. However, accurate high-throughput methods to detect and quantify oligomer populations are still needed. We present here a single-molecule approach for the detection and quantification of oligomeric species. The approach is based on the use of solid-state nanopores and multiplexed DNA barcoding to identify and characterize oligomers from multiple samples. We study α-synuclein oligomers in the presence of several small-molecule inhibitors of α-synuclein aggregation as an illustration of the potential applicability of this method to the development of diagnostic and therapeutic methods for Parkinson's disease.
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