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Published on: May 27, 2011
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Artificial COVID-19 T-Cell Immunogen.
M B Borgoyakova1, L I Karpenko2, A P Rudometov2
1State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia. borgoyakova_mb@vector.nsc.ru.
Bulletin of Experimental Biology and Medicine
|November 18, 2023
Summary
Researchers engineered an artificial T-cell immunogen using conserved SARS-CoV-2 protein fragments. This novel antigen successfully induced cellular immunity in mice, demonstrating its potential for vaccine development.
Area of Science:
- Immunology
- Virology
- Biotechnology
Background:
- The SARS-CoV-2 virus poses a significant global health threat.
- Developing effective vaccines that elicit robust cellular immunity is crucial for controlling viral infections.
- T-cell based immunogens are essential for long-term immune memory and protection.
Purpose of the Study:
- To design and characterize a novel artificial T-cell immunogen based on conserved SARS-CoV-2 epitopes.
- To evaluate the immunogenic properties of this artificial immunogen in a preclinical mouse model.
- To assess the expression and processing of the engineered immunogen in vitro and in vivo.
Main Methods:
- Construction of an artificial gene encoding a multi-epitope immunogen from conserved SARS-CoV-2 proteins.
- Cloning the immunogen gene into the pVAX1 plasmid, with and without an N-terminal ubiquitin tag.
- In vitro expression analysis in HEK293T cells.
- In vivo immunogenicity studies in BALB/c mice.
Main Results:
- The engineered immunogen protein was successfully synthesized and expressed in HEK293T cells.
- Plasmids containing the artificial immunogen gene demonstrated effective protein production both in vitro and in vivo.
- Administration of the artificial immunogen induced a significant cellular immune response in BALB/c mice.
Conclusions:
- The developed artificial T-cell immunogen is actively processed and immunogenic.
- This approach offers a promising strategy for developing next-generation vaccines against SARS-CoV-2.
- The artificial immunogen has the potential to induce protective cellular immunity.
