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Updated: Jul 10, 2025

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
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Influence of Melatonin on Maturation of Male Germ Cells

S M Slesarev1, A P Okaemova2, M E Diatroptov3

  • 1Ulyanovsk State University, Ulyanovsk, Russia. sergey_sl@mail.ru.

Bulletin of Experimental Biology and Medicine
|November 18, 2023
PubMed

Insights

Epiphysectomy damages male germ cell DNA and disrupts enzyme rhythms. Melatonin administration reverses this damage, protecting DNA and restoring normal enzyme activity in rats.

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Chronobiology

Background:

  • Epiphysectomy (pinealectomy) impacts reproductive health.
  • Circadian rhythms are crucial for cellular processes, including DNA repair.
  • Male germ cell DNA integrity is vital for fertility.

Purpose of the Study:

  • To investigate the effects of epiphysectomy on male germ cell DNA damage.
  • To examine the impact of epiphysectomy on the daily rhythms of DNA repair enzymes.
  • To determine if melatonin administration can mitigate epiphysectomy-induced damage and restore circadian rhythms.

Main Methods:

  • Studied outbred white rats undergoing epiphysectomy.
  • Administered melatonin to epiphysectomized rats.
  • Assessed DNA damage levels in developing male germ cells.
  • Monitored daily activity dynamics of PARP-1 and caspase-3 enzymes.

Main Results:

  • Epiphysectomy increased DNA damage in male germ cells.
  • Circadian rhythms of PARP-1 and caspase-3 activity were abolished post-epiphysectomy.
  • Melatonin treatment reduced DNA damage levels.
  • Melatonin restored the circadian rhythmicity of PARP-1 and caspase-3 activity.

Conclusions:

  • Epiphysectomy negatively affects male germ cell DNA integrity and circadian enzyme regulation.
  • Melatonin shows potential as a protective agent for male gamete DNA.
  • Melatonin may indirectly protect maturing male gametes by restoring circadian rhythms.
Keywords:
DNA protectioncircadian rhythmepiphysectomymale germ cellsmelatonin

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