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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 17, 2026

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells
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Gold-Nanorod-Assisted Live Cell Nuclear Imaging Based on Near-Infrared II Dark-Field Microscopy.

Yifeng Shi1, Shiyi Peng2, Zhongyu Huang1

  • 1Key Laboratory of Optical Information Detection and Display Technology of Zhejiang, Zhejiang Normal University, Jinhua 321004, China.

Biology
|November 24, 2023
PubMed
Summary
This summary is machine-generated.

This study demonstrates superior dark-field imaging of colorectal cancer cells using the NIR-II window. Gold nanorods enhanced imaging contrast, showing potential for precise cancer cell labeling and targeted therapies.

Keywords:
dark-field microscopygold nanorodslive cell nuclear imagingnear-infrared second window

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Area of Science:

  • Biomedical Optics
  • Nanotechnology
  • Cancer Research

Background:

  • Dark-field microscopy offers high contrast and minimal cell damage for cell imaging.
  • The Near-Infrared II (NIR-II) window provides low autofluorescence and high signal-to-background ratio (SBR) for optical imaging.
  • Colorectal cancer imaging requires advanced techniques for improved contrast and specificity.

Purpose of the Study:

  • To evaluate dark-field microscopy in the NIR-II window for colorectal cancer cell imaging.
  • To synthesize and utilize gold nanorods (GNRs) for enhanced NIR-II dark-field imaging.
  • To explore targeted nuclear labeling of cancer cells for potential therapeutic applications.

Main Methods:

  • Comparison of dark-field imaging in visible and NIR-II wavelengths for colorectal cancer cells.
  • Synthesis of GNRs with localized surface plasmon resonance (LSPR) in the NIR-II window.
  • Non-specific and specific labeling of colorectal cancer cells using modified GNRs for NIR-II dark-field scattering imaging.

Main Results:

  • NIR-II dark-field imaging demonstrated superior performance compared to visible wavelengths.
  • GNR-labeled cells showed a sixfold increase in SBR, particularly in the 1425-1475 nm range.
  • Specific nuclear labeling of cancer cells achieved higher SBR and precise probe localization.

Conclusions:

  • NIR-II dark-field imaging with GNRs significantly enhances contrast for colorectal cancer cell visualization.
  • The developed imaging system shows promise for precise cancer cell labeling.
  • This technology holds potential for applications in photothermal therapy and drug delivery for cancer treatment.