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Related Concept Videos

Flow Cytometry01:23

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
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Optofluidic Flow Cytometer with In-Plane Spherical Mirror for Signal Enhancement.

Filippo Zorzi1,2, Silvio Bonfadini1, Ludovico Aloisio1,2

  • 1Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, 20134 Milan, Italy.

Sensors (Basel, Switzerland)
|November 25, 2023
PubMed
Summary
This summary is machine-generated.

A novel optofluidic flow cytometer with an integrated mirror enhances signal collection for detecting small microparticles like cells and bacteria. This technology improves signal-to-noise ratio for point-of-care applications.

Keywords:
FLICELab on a Chipfemtosecond laser microfabricationflow cytometryoptofluidic particles detection

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Optical Engineering

Background:

  • Single microparticle analysis is crucial for fields like cell biology and diagnostics.
  • Traditional flow cytometry instruments are often large, costly, and unsuitable for point-of-care (PoC) use.
  • Existing microfluidic flow cytometers face challenges in detecting small particles due to complex designs and reliance on external optics.

Purpose of the Study:

  • To develop a compact and cost-effective optofluidic flow cytometer for enhanced microparticle detection.
  • To improve the signal-to-noise ratio for analyzing small particles, including cells and bacteria.
  • To enable simultaneous collection of fluorescence and scattering signals for better particle characterization.

Main Methods:

  • Integration of a 3D in-plane spherical mirror into a microfluidic flow cytometer for improved optical signal collection.
  • Utilizing a single optical fiber for simultaneous detection of particle fluorescence and scattering.
  • Characterization of the device performance using fluorescent polystyrene beads of varying sizes.

Main Results:

  • Achieved a six-fold increase in signal-to-noise ratio, enabling detection of particles as small as 1.5 µm.
  • Demonstrated simultaneous collection of fluorescence and scattering signals, facilitating differentiation of particle populations.
  • Successfully analyzed signals from fluorescent HEK cells and *Escherichia coli* bacteria as a proof of concept.

Conclusions:

  • The developed optofluidic flow cytometer offers a promising solution for sensitive and efficient microparticle analysis.
  • The integrated spherical mirror design significantly enhances optical signal collection, overcoming limitations of previous microfluidic systems.
  • This technology holds potential for point-of-care applications, enabling on-site analysis of biological samples.