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Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines
Published on: June 1, 2022
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Developing Wolbachia-Infected Cell Lines from Insects
Mukund Madhav1,2, Peter James3
1Liverpool School of Tropical Medicine, Liverpool, UK.
Methods in Molecular Biology (Clifton, N.J.)
|November 25, 2023
Abstract
None:
Wolbachia, an intracellular bacterium of arthropods, is an ideal candidate for use in the biological control of insect pests. The inability of Wolbachia to grow in the extracellular environment requires maintenance in live insects or insect cell cultures. Growing and adapting high-density Wolbachia in the targeted host cells improves the possibility of cross-species transinfection. Here, we describe a method for the establishment of a primary cell culture from insect embryos and its transinfection with Wolbachia.
