Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Seeing lipids where they live.

Nature cell biology·2026

Same author

PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.

Nature communications·2025

Same author

Nano-org, a functional resource for single-molecule localisation microscopy data.

Nature communications·2025

Same author

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Nature methods·2025

Same author

[Diving into the heart of axonal endocytosis].

Medecine sciences : M/S·2025

Same author

Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues.

Nature protocols·2025

Same journal

Deep learning in tumour genomics: from multi-omics integration to precision oncology.

Open biology·2026

Same journal

Understanding GnRH: local systems, signalling mechanisms and implications in female health.

Open biology·2026

Same journal

The evolution and functional significance of neuropeptide cocktails: insights from SALMFamides in asteroid echinoderms.

Open biology·2026

Same journal

Structural basis of Drosophila insulin receptor activation by DILP2 hormone.

Open biology·2026

Same journal

Parental rearing shapes brain functional networks and socio-sexual behaviours in the prairie vole.

Open biology·2026

Same journal

The periosteum as an endocrine organ: historical foundations and new insights.

Open biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Decoding life's inner workings: advances in quantitative bioimaging.

Ricardo Henriques^1,2, Christophe Leterrier³, Aubrey Weigel⁴

¹Optical cell biology group, Instituto Gulbenkian de Ciência, Oerias, Portugal.

|November 29, 2023

Summary

This summary is machine-generated.

Quantitative bioimaging advances enable nanoscale visualization of cellular processes. These powerful techniques offer new insights into molecular structures and dynamic cell functions, revolutionizing biological understanding.

Keywords:

fluorescence imaging machine learning morphological characterization quantitative bioimaging single-molecule tracking super-resolution microscopy

More Related Videos

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

Published on: August 4, 2022

Related Experiment Videos

Last Updated: Jun 21, 2026

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

Published on: August 4, 2022

Area of Science:

Cellular Biology
Microscopy
Biophysics

Background:

Quantitative bioimaging integrates advanced microscopy with computational analysis for precise measurement of biological structures and processes.
Techniques like super-resolution microscopy and single-particle analysis provide nanoscale visualization of molecular and cellular dynamics.

Discussion:

The special feature explores diverse applications, from mapping nuclear pore proteins and tracking histone deposition to correlating calcium release with protein organization in heart cells.
It also covers tools for analyzing microglia morphology changes under various conditions, demonstrating the breadth of quantitative bioimaging applications.

Key Insights:

Cutting-edge quantitative imaging techniques offer unprecedented insights into complex molecular structures and dynamic cellular processes *in situ*.
Precise measurement of biological structures and processes is achieved through the synergy of advanced imaging and computational analysis.

Outlook:

Future progress depends on addressing challenges in molecular probes, algorithms, and correlation protocols to fully harness the potential of these technologies.
Continued advancements in quantitative bioimaging promise to further revolutionize our understanding of living systems.