Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High Pressure Synthesis of Ultrasmall Nanodiamonds with Nitrogen Vacancy Centers.

Nano letters·2026

Same author

Staging and defect-limited intercalation of FeCl<sub>3</sub> in graphite electrodes.

Nature communications·2026

Same author

Engineering <i>Escherichia coli</i> for Urease-Driven Synthesis of Metal Oxide Nanomaterials.

ACS synthetic biology·2026

Same author

Unsupervised Segmentation and Clustering Workflow for Efficient Processing of 4D-STEM and 5D-STEM Data.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026

Same author

Polyolefin blends with co-continuous architectures enabled by dynamic covalent crosslinking.

Science advances·2026

Same author

Relaxing Direct Ptychography Sampling Requirements via Parallax Imaging Insights.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026

Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026

Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026

Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026

Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026

Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026

Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 9, 2025

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

Solving complex nanostructures with ptychographic atomic electron tomography.

Philipp M Pelz^1,2,3, Sinéad M Griffin^4,5, Scott Stonemeyer^5,6,7,8

¹Institute of Micro- and Nanostructure Research (IMN) & Center for Nanoanalysis and Electron Microscopy (CENEM), Friedrich Alexander-Universität Erlangen-Nürnberg, IZNF, 91058, Erlangen, Germany. philipp.pelz@fau.de.

Nature Communications

|November 30, 2023

Summary

This summary is machine-generated.

Ptychographic electron tomography reconstructs atomic structures in nanomaterials. This new method reveals a previously unknown ZrTe2 phase within a carbon nanotube, advancing materials science and structural biology.

More Related Videos

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Related Experiment Videos

Last Updated: Jul 9, 2025

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Area of Science:

Materials Science
Structural Biology
Nanotechnology

Background:

Transmission electron microscopy (TEM) is crucial for atomic-scale structural determination.
Current methods like phase-contrast TEM and atomic electron tomography (AET) have limitations for heterogeneous nanomaterials with light elements.

Purpose of the Study:

To develop and apply a novel ptychographic electron tomography technique for atomic resolution imaging.
To determine the three-dimensional atomic structure of a complex nanomaterial containing light elements.

Main Methods:

Acquisition of 34.5 million diffraction patterns using ptychographic electron tomography.
Reconstruction of an atomic resolution tilt series of a double wall-carbon nanotube (DW-CNT).
Class averaging and subpixel localization of atomic peaks.

Main Results:

Successful reconstruction of the atomic structure of a DW-CNT encapsulating a ZrTe sandwich structure.
Identification of a Zr11Te50 structure containing a novel ZrTe2 phase in the core.
Demonstration of atomic resolution imaging for beam-sensitive nanomaterials.

Conclusions:

Ptychographic electron tomography enables atomic resolution structural determination of complex, heterogeneous nanomaterials.
The study reveals a new ZrTe2 phase, expanding knowledge of zirconium telluride systems.
This technique opens new avenues for analyzing light-element nanomaterials in various scientific fields.