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Methods to Assess Microbial Populations

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Updated: Jun 13, 2026

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

Published on: May 30, 2014

Microplate-Based Cell Viability Assay as a Cost-Effective Alternative to Flow Cytometry for Microalgae Analysis.

Diyuan Wang¹, Francis L de Los Reyes¹, Joel J Ducoste¹

¹Department of Civil, Construction, and Environmental Engineering, North Carolina State University, Raleigh, North Carolina 27606, United States.

Environmental Science & Technology

|December 4, 2023

Summary

This summary is machine-generated.

New microplate assays offer a low-cost, simple method for assessing microalgae cell viability, crucial for biorefinery and bioremediation applications. This technique provides reliable results comparable to expensive flow cytometry.

Keywords:

Dunaliella viridis cell viability erythrosine B fluorescein diacetate (FDA)fluorescent dye microalgae microplate reader

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High-Throughput Metabolic Profiling for Model Refinements of Microalgae

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

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Last Updated: Jun 13, 2026

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

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Published on: May 30, 2014

A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated WATER-PAM Fluorometry

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High-Throughput Metabolic Profiling for Model Refinements of Microalgae

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

Area of Science:

Biotechnology
Microalgae Cultivation
Cell Biology

Background:

Cell viability is essential for microalgae cultivation in biorefineries and bioremediation.
Current flow cytometry methods for viability assessment are expensive and impractical for industrial use.
Development of cost-effective and accessible viability assays is needed.

Approach:

Developed novel microplate assays using fluorescein diacetate (live stain) and erythrosine B (dead stain).
Validated assays by monitoring *Dunaliella viridis* viability under nutrient stress, comparing results with flow cytometry.
Systematically investigated assay parameters: dye concentration, incubation time, and background fluorescence.

Key Points:

Microplate assays provide a low-cost, simple, and reliable alternative to flow cytometry for microalgae viability.
Assays accurately tracked *Dunaliella viridis* viability under carbon and nitrogen limitation.
Comprehensive review of microalgae staining dyes and protocol optimization strategies included.

Conclusions:

The developed microplate assays are suitable for industrial applications in microalgae cultivation.
This method offers valuable insights for future microalgae viability assessments and applications.
Provides a practical tool for quality control in biorefinery and bioremediation processes.