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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Updated: Jul 9, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications
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Published on: April 7, 2023

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Fluorescence lifetime Hong-Ou-Mandel sensing.

Ashley Lyons1, Vytautas Zickus2,3, Raúl Álvarez-Mendoza2

  • 1School of Physics and Astronomy, University of Glasgow, Glasgow, G12 8QQ, UK. ashley.lyons@glasgow.ac.uk.

Nature Communications
|December 4, 2023
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Summary
This summary is machine-generated.

This study introduces a new fluorescence lifetime measurement technique achieving picosecond resolution, significantly improving upon current methods. This advancement enables the study of fast biological processes and material interactions.

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Area of Science:

  • Optics and Photonics
  • Biophysics
  • Materials Science

Background:

  • Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) traditionally relies on electronic time tagging or gated detectors.
  • Current FLIM techniques are limited by electronic performance, restricting temporal resolution to hundreds of picoseconds.

Purpose of the Study:

  • To develop a novel fluorescence lifetime measurement technique with significantly enhanced temporal resolution.
  • To demonstrate the capability of this technique for precise measurements of short fluorescence lifetimes.
  • To introduce a new method for contact-free nanorheology and explore applications in studying fast biological processes.

Main Methods:

  • Utilized photon-bunching statistics for fluorescence lifetime measurements.
  • Achieved picosecond timescale resolution, dependent only on reference pulse duration.
  • Measured fluorescence lifetimes of various dyes (1.6–7 ps) within ~1 second.

Main Results:

  • Demonstrated a temporal resolution of 1–0.1 picoseconds, surpassing conventional electronic methods.
  • Successfully measured fluorescence lifetimes of multiple dyes with high accuracy and speed.
  • Validated the technique by measuring glycerol/water mixture viscosity using a molecular rotor, showcasing nanorheology applications.

Conclusions:

  • The developed photon-bunching technique offers unprecedented temporal resolution for fluorescence lifetime measurements.
  • This method opens new avenues for investigating ultrafast biological dynamics and fundamental photophysical interactions.
  • The technique provides a novel platform for contact-free nanorheology and advanced material characterization.