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Related Concept Videos

CRISPR01:59

CRISPR

51.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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Programmable Aptasensor for Regulating CRISPR/Cas12a Activity.

Yao Yu1, Qiaoyu Li2,3, Wen Shi1,2

  • 1College of Chemistry & Pharmacy, Northwest A&F University Yangling, Shaanxi 712100, China.

ACS Sensors
|December 12, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a CRISPR-mediated programmable aptasensor (CPAS) for detecting non-nucleic acid targets. The CPAS platform offers a customizable and sensitive approach for rapid diagnostics, demonstrated for SARS-CoV-2 spike protein and ATP.

Keywords:
CRISPR/Cas12aaptamersbiosensorsmolecular recognitionpoint-of-care testing

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biosensing

Background:

  • CRISPR-mediated aptasensors are increasingly used for non-nucleic acid target detection.
  • Existing designs require complex modifications for improved performance and broader applications.

Purpose of the Study:

  • To develop a versatile and easily customizable CRISPR-mediated programmable aptasensor (CPAS) platform.
  • To enhance signal-to-noise ratio, universality, and detection range for aptasensors.

Main Methods:

  • A CPAS platform was designed using aptamer, locker DNA, and crRNA recognition region in a hairpin structure.
  • T4 DNA polymerase and Cas12a enzyme were utilized to generate fluorescence signals upon target binding.
  • The platform's customization was achieved by altering aptamer and locker DNA sequences.

Main Results:

  • The CPAS platform demonstrated tunable locker DNA lengths for different targets (7 nt for S protein, 4 nt for ATP).
  • High sensitivity was achieved for detecting SARS-CoV-2 spike (S) protein and adenosine triphosphate (ATP).
  • Integration with lateral flow assay allowed for sensitive detection within 1 hour.

Conclusions:

  • The CPAS platform provides a highly customizable and sensitive method for detecting non-nucleic acid targets.
  • Its design facilitates rapid, portable diagnostics with potential applications in various fields.
  • The platform shows promise for point-of-care testing and molecular diagnostics.