Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pathophysiology of Heart Failure01:17

Pathophysiology of Heart Failure

1.6K
Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...
1.6K
Electrophysiology of Normal Cardiac Rhythm01:19

Electrophysiology of Normal Cardiac Rhythm

4.7K
The normal cardiac rhythm is a synchronized electrical activity that facilitates the regular and coordinated contraction of the heart muscle. This process is essential for efficient blood circulation throughout the body. The fundamental elements involved in establishing and maintaining this rhythm include the unique electrical properties of cardiac muscle cells, the sinoatrial (SA) node's pacemaker function, the specialized conducting system, and the ionic mechanisms underlying each phase...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mural cells protect the adult brain from hemorrhage but do not control the blood-brain barrier in developing zebrafish.

eLife·2026
Same author

A scalable, multi-resolution consensus clustering approach for prioritizing robust signals from high-throughput screens.

Briefings in bioinformatics·2026
Same author

Application of spatial transcriptomics across organoids for a high-resolution, spatial whole-transcriptome benchmarking dataset.

iScience·2026
Same author

Identifying therapeutic targets in low-grade serous ovarian carcinomas with no specific molecular profile.

The Journal of pathology·2026
Same author

Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects.

Cell·2026
Same author

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes.

Nature communications·2026

Related Experiment Video

Updated: Jul 8, 2025

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

30.3K

HOPX-associated molecular programs control cardiomyocyte cell states underpinning cardiac structure and function.

Clayton E Friedman1, Seth W Cheetham2, Sumedha Negi1

  • 1Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.

Developmental Cell
|December 13, 2023
PubMed
Summary

The homeodomain protein HOPX regulates cardiac gene networks essential for heart development and function. Cell signaling pathways control HOPX, impacting cardiomyocyte identity and regeneration in disease models.

Keywords:
CRISPRiDamIDcardiomyocyte physiologycell proliferationcomplex traitsheart developmenthuman-induced pluripotent stem cellmaturationregenerationzebrafish cardiac regeneration

More Related Videos

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts
09:29

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Published on: March 22, 2017

7.5K
An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
06:02

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

Published on: November 2, 2020

5.8K

Related Experiment Videos

Last Updated: Jul 8, 2025

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

30.3K
Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts
09:29

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Published on: March 22, 2017

7.5K
An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
06:02

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

Published on: November 2, 2020

5.8K

Area of Science:

  • Cardiovascular Biology
  • Stem Cell Biology
  • Genomics

Background:

  • Cardiac development relies on precise genomic regulation of cardiomyocyte differentiation.
  • The role of non-DNA-binding proteins like HOPX in this process is not fully understood.

Purpose of the Study:

  • To investigate the genomic regulatory function of the homeodomain protein HOPX in cardiomyocyte differentiation and function.
  • To elucidate the upstream regulators and downstream targets of HOPX in cardiac gene programs.

Main Methods:

  • Utilized human-induced pluripotent stem cell-derived cardiomyocytes for loss-of-function studies.
  • Performed in vitro perturbation studies to analyze cell growth and proliferation effects on HOPX.
  • Employed cell, organoid, and zebrafish regeneration models to assess HOPX function in development and disease.

Main Results:

  • HOPX interacts with and regulates cardiac genes and enhancer networks crucial for heart development.
  • Upstream cell growth and proliferation signals modulate HOPX transcription, thereby controlling cardiac gene programs.
  • HOPX-regulated gene programs are critical for cardiomyocyte function during development and in disease contexts.

Conclusions:

  • Mechanistically links cell signaling pathways to HOPX transcription as a key regulator of cardiomyocyte identity.
  • Demonstrates HOPX's vital role in controlling gene programs essential for cardiac development, function, and regeneration.