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Related Experiment Video

Updated: Jul 1, 2026

Isolation and Culture of Mouse Cortical Astrocytes

Isolation and Culture of Mouse Cortical Astrocytes

Published on: January 19, 2013

BACH1 changes microglial metabolism and affects astrogenesis during mouse brain development.

Yanyan Wang¹, Wenwen Wang², Libo Su¹

¹State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Beijing Institute for Stem Cell and Regenerative Medicine, Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.

Developmental Cell

|December 15, 2023

Summary

Microglia metabolism impacts brain development. Bach1 maintains microglial metabolic homeostasis, influencing astrogenesis and neuronal differentiation, crucial for preventing neurodevelopmental disorders.

Keywords:

BACH1 LRRC15 astrogenesis histone lactylation microglia metabolism

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Area of Science:

Neuroscience
Immunology
Developmental Biology

Background:

Microglia, the resident immune cells of the central nervous system, exhibit significant heterogeneity.
Microglial metabolic reprogramming is linked to proinflammatory states in disease, but its role in embryonic development and influence on astrogenesis remain unclear.

Purpose of the Study:

To elucidate the communication between microglial metabolism and astrogenesis during embryonic brain development.
To investigate the role of the transcription factor Bach1 in regulating microglial metabolism and its downstream effects.

Main Methods:

Analysis of microglial glycolysis and lactate production, focusing on enzymes HK2 and GAPDH.
Investigation of the interaction between microglia-derived LRRC15 and CD248 in the JAK/STAT pathway.
Utilizing Bach1 conditional knockout (cKO) mice (Bach1^cKO-Cx3) to assess in vivo effects.

Main Results:

Bach1 inhibits glycolysis by suppressing HK2 and GAPDH, reducing microglial lactate production.
Metabolic changes in microglia alter histone modifications at the Lrrc15 promoter.
Microglia-derived LRRC15, via CD248 and the JAK/STAT pathway, influences astrogenesis.
Bach1 deficiency in microglia leads to abnormal neuronal differentiation and anxiety-like behaviors in mice.

Conclusions:

Microglial metabolic homeostasis is critical for proper astrogenesis during early brain development.
Dysregulation of microglial metabolism can impact neuronal development and behavior.
This study provides novel insights into the interplay between microglia and astrocytes, relevant to neurodevelopmental disorders.