Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

792
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
792
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

1.0K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
1.0K
Mass Spectrometers01:16

Mass Spectrometers

5.5K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.5K
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

749
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
749
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.5K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging roles of ferroptosis in modulating the immune landscape of glial tumours.

Nature cell biology·2026
Same author

MiR-940 Suppresses Ferroptosis by Controlling Expression of Key Regulatory Genes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

GPX4 regulates lipid peroxidation and ferroptosis of stored red blood cells.

Blood. Red cells & iron·2026
Same author

Dissecting Polypharmacology in Phenotypic Screening to Resolve Ferroptotic and Necrotic Cell-Death Mechanisms.

ACS medicinal chemistry letters·2026
Same author

When Pathways Converge: Iron, Lipid Peroxidation, and α-Synuclein in Ferroptosis-Driven Dopaminergic Neurodegeneration.

Journal of neurochemistry·2026
Same author

Ferroptosis as an approach to leverage cancer metabolism.

Trends in cell biology·2026

Related Experiment Video

Updated: Jul 8, 2025

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI
08:02

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

Published on: December 16, 2022

1.9K

Spatial pharmacology using mass spectrometry imaging.

Presha Rajbhandari1, Taruna V Neelakantan2, Noreen Hosny3

  • 1Department of Biological Sciences, Columbia University, New York, NY, USA.

Trends in Pharmacological Sciences
|December 16, 2023
PubMed
Summary

Spatial pharmacology uses mass spectrometry imaging (MSI) to map drug distribution and effects without labeling. This technology offers new insights for drug discovery and development by revealing spatial pharmacokinetics and biomarkers.

Keywords:
DESMALDISIMSimagingmass spectrometry imagingmultimodal imaging

More Related Videos

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
07:05

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

Published on: June 20, 2025

471
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.7K

Related Experiment Videos

Last Updated: Jul 8, 2025

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI
08:02

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

Published on: December 16, 2022

1.9K
Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
07:05

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

Published on: June 20, 2025

471
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.7K

Area of Science:

  • Spatial biology
  • Pharmacology
  • Biomolecular analysis

Background:

  • Mass spectrometry imaging (MSI) is a powerful technology for analyzing the spatial distribution of molecules within biological tissues.
  • Current applications of MSI are expanding into drug discovery and development, offering new insights into drug behavior and effects.
  • Understanding the spatial distribution of drugs and their interactions with endogenous biomolecules is crucial for effective therapeutic development.

Purpose of the Study:

  • To provide a perspective on implementing mass spectrometry imaging (MSI) technologies and computational tools for spatial pharmacology.
  • To highlight the potential of comprehensive spatial pharmacology through multimodal MSI data integration with other spatial technologies.
  • To address challenges in reproducibility and compound annotation for robust conclusions in drug discovery and development.

Main Methods:

  • Utilizing mass spectrometry imaging (MSI) to map the spatial distribution of drugs and their metabolites.
  • Analyzing the effects of drugs on endogenous biomolecules, including metabolites, lipids, proteins, peptides, and glycans, without labeling.
  • Integrating multimodal MSI data with other spatial technologies for a comprehensive understanding.

Main Results:

  • MSI enables the mapping of drug distribution and effects on endogenous biomolecules in a label-free manner.
  • Quantitative spatial drug pharmacokinetics, toxicology, tissue subtyping, and associated biomarkers can be revealed.
  • Multimodal MSI data integration offers comprehensive spatial pharmacology insights.

Conclusions:

  • Spatial pharmacology, powered by MSI, provides unprecedented insights into drug behavior and effects within tissues.
  • The integration of MSI with computational tools and other spatial technologies is key to advancing drug discovery and development.
  • Addressing challenges in reproducibility and annotation will enhance the reliability and impact of spatial pharmacology studies.